Tracking of Leptin, Soluble Leptin Receptor, and the Free Leptin Index during Weight Loss and Regain in Children

Tracking of leptin, soluble leptin receptor, and the free leptin index during weight loss and regain in children.

OBJECTIVE To investigate changes in leptin and soluble leptin receptor (SLR) concentrations, and in the free leptin index (FLI) during weight loss and subsequent weight regain; and to ascertain whether these indices remain stable in the rank of the distribution in repeated measures (tracking) during perturbations of weight in obese children. DESIGN AND MEASUREMENTS In a longitudinal study, 11...

Longitudinal analysis of leptin variation during weight regain after weight loss in obese children.

OBJECTIVE This study assessed if lower than predicted serum leptin concentrations seen during weight loss persisted during weight regain, with possible implications for weight control. METHODS 115 children were investigated during a 12-week weight loss program. 90 children completed the program, and 68 children entered a follow-up program spanning 28 months. Measurements were performed at bas...

Detection of leptin and leptin receptor mRNA expression in the bovine t

Leptin and soluble leptin receptor levels in obese and weight-losing individuals.

To investigate soluble leptin receptor (sLR) in plasma, specific anti-sLR monoclonal antibodies were developed. Western blot analysis and size exclusion fractionation demonstrated sLR in plasma with a molecular mass of approximately 180,000. Next to this, the presence of sLR-leptin complexes in plasma was confirmed. Using the developed monoclonal antibodies, a specific sLR ELISA was developed, ...

Expression of leptin and leptin receptor transcript in ovine corpus luteum

Background: Leptin, the product of the obesity (ob) gene, acts as a signaling adipokine for modulating food intake, energy metabolism and reproductive functions in mammals. Leptin’s effects on the reproductive system at various levels of the hypothalamic-pituitary-gonadal axis have been established. Moreover, the direct and local effect of leptin on bovine oocyte maturation and corpus luteum fu...